Composite
Part:BBa_K2923020:Design
Designed by: Kateryna LEN Group: iGEM19_Strasbourg (2019-10-13)
MS2-MS2-Guanine actif aptazyme-PP7-PP7
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 25
Illegal PstI site found at 64 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was created by Gibson assembly.
Source
This present part is composed of two MS2 (from Escherichia phage MS2) and two PP7 (Pseudomonas phage PP7) stem-loops. The guanine aptazyme sequence is previously described by Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.
References
Berry, K.E., and Hochschild, A. (2018). A bacterial three-hybrid assay detects Escherichia coli Hfq-sRNA interactions in vivo. Nucleic Acids Research 46 Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.